{"id":3962,"date":"2019-11-08T12:33:43","date_gmt":"2019-11-08T11:33:43","guid":{"rendered":"https:\/\/site.uit.no\/cage\/?p=3962"},"modified":"2023-04-04T10:31:08","modified_gmt":"2023-04-04T08:31:08","slug":"always-be-afraid-of-dna-contamination","status":"publish","type":"post","link":"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/","title":{"rendered":"Always be afraid of DNA contamination!"},"content":{"rendered":"<p>Text and photos: Danielle Grant and Kristine Steinsland, <a href=\"http:\/\/norceresearch.no\">NORCE Climate<\/a><\/p>\n<p>Danielle and Kristine here! We are two PhD students from NORCE Climate, based in Bergen.<\/p>\n<p>Our research is part of the larger project, <a href=\"https:\/\/www.agensi.eu\/\">AGENSI<\/a>: A Genetic View into Past Sea Ice Variability in the Arctic. The Arctic is drastically changing and there may soon be summer without sea ice. However, the impacts of a warming Arctic on regional and global climate systems are not well understood. We can use knowledge of past sea ice variability to understand the role of sea ice on Arctic climate, and learn more about our current situation. To do this we do sea ice reconstructions based on observational data (satellite and historical records) and sea ice proxies.<\/p>\n<figure id=\"attachment_3964\" aria-describedby=\"caption-attachment-3964\" style=\"width: 768px\" class=\"wp-caption aligncenter\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-3964 size-large\" src=\"https:\/\/site.uit.no\/cage\/wp-content\/uploads\/2019\/11\/agensi_sea-ice-768x1024.jpg\" alt=\"\" width=\"768\" height=\"1024\" srcset=\"https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/agensi_sea-ice-768x1024.jpg 768w, https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/agensi_sea-ice-225x300.jpg 225w, https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/agensi_sea-ice-1920x2560.jpg 1920w, https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/agensi_sea-ice.jpg 1536w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/><figcaption id=\"caption-attachment-3964\" class=\"wp-caption-text\">To understand the role of sea ice on the Arctic climate we reconstruct past changes in sea ice based on observational data (satellite and historical records) and sea ice proxies. Photo: AGENSI<\/figcaption><\/figure>\n<p>Our main goal on board is to collect surface sediment samples from multicores\u2014which we will analyze back in Bergen for environmental DNA, IP25 biomarker, and palynology. Environmental DNA is an emerging tool for sea ice reconstructions, spearheaded by the AGENSI group. As we build confidence in this new proxy the surface samples collected during this cruise will contribute to a surface sediment database. The database will provide a calibration for our suite of proxies, which can then be applied for paleo-reconstructions of sea ice (hopefully on a core ranging back 150,000 years from this cruise)!<\/p>\n<p>After 20 days onboard we have collected 32 multicores ranging from 100m depth to 2500 m. When we process a core, we first clean away the mud, and then wash with a chlorine solution (This is the first of many cleaning steps!) The core is then delivered to the molecular lab to one of us already dressed in a paper suit, hairnet, particle mask, safety glasses and double gloves! The core is then immediately cleaned with chlorine\u2026 again\u2026twice. These cleaning steps are critical to ensure no DNA contamination! Always be afraid of DNA contamination!<\/p>\n<p>Once things are clean, we can then take our surface samples using sterile instruments. Sampling the surface of a multicore is quite exciting! The MC preserves the sediment-water interface, meaning that you get an intact slice of the sea floor. So far, we have encountered plenty of tube worms, burrowing worms, and even a lively starfish!<\/p>\n<figure id=\"attachment_3965\" aria-describedby=\"caption-attachment-3965\" style=\"width: 1024px\" class=\"wp-caption aligncenter\"><img decoding=\"async\" class=\"wp-image-3965 size-large lazyload\" data-src=\"https:\/\/site.uit.no\/cage\/wp-content\/uploads\/2019\/11\/agensi_cleaning02-copy-1024x370.jpg\" alt=\"\" width=\"1024\" height=\"370\" data-srcset=\"https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/agensi_cleaning02-copy-1024x370.jpg 1024w, https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/agensi_cleaning02-copy-300x108.jpg 300w, https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/agensi_cleaning02-copy-768x278.jpg 768w, https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/agensi_cleaning02-copy.jpg 1601w\" data-sizes=\"(max-width: 1024px) 100vw, 1024px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 1024px; --smush-placeholder-aspect-ratio: 1024\/370;\" \/><figcaption id=\"caption-attachment-3965\" class=\"wp-caption-text\">Cleaning the samples is crucial to collect pristine DNA. Photo: AGENSI<\/figcaption><\/figure>\n<p>For some locations we also do a full downcore sampling, meaning we sample every 2cm of a split core. We like to think of this as \u2018sediment surgery\u2019\u2014we have another designated clean lab exclusively for DNA downcore samples. The different levels of clean would not be possible without being on board KPH, it is a great luxury to have such control on the lab space and really limit any chance of contamination.<\/p>\n<p>When we aren\u2019t sampling (or cleaning), we can usually be found in the mess room (the juice on board is amazing) or on the observation deck with coffee! The coffee may be just as important as all the cleaning for our success!<\/p>\n<p>The last few days have been exciting and busy with a lot of coring sites. We were slow to realize just how dark things have gotten outside! As things get darker and colder, we plan to visit the ship\u2019s sauna we hear so such wonderful things about! Until then, happy cleaning and stay cool\u2026 like sea ice!<\/p>\n<p><a href=\"https:\/\/www.agensi.eu\/\">If you\u2019d like to read more about AGENSI, visit agensi.eu!<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Text and photos: Danielle Grant and Kristine Steinsland, NORCE Climate Danielle and Kristine here! We &hellip; <\/p>\n<p class=\"read-more-link\"><a href=\"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/\">Read more<span class=\"screen-reader-text\"> \u00abAlways be afraid of DNA contamination!\u00bb<\/span><\/a><\/p>\n","protected":false},"author":123546,"featured_media":3963,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[121,120],"tags":[],"class_list":["post-3962","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-calypso-coring-in-the-fram-strait-19-3","category-cruise-blog"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Always be afraid of DNA contamination! - CAGE, Centre for Arctic Gas Hydrate, Environment and Climate<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Always be afraid of DNA contamination! - CAGE, Centre for Arctic Gas Hydrate, Environment and Climate\" \/>\n<meta property=\"og:description\" content=\"Text and photos: Danielle Grant and Kristine Steinsland, NORCE Climate Danielle and Kristine here! We &hellip; Read more \u00abAlways be afraid of DNA contamination!\u00bb\" \/>\n<meta property=\"og:url\" content=\"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/\" \/>\n<meta property=\"og:site_name\" content=\"CAGE, Centre for Arctic Gas Hydrate, Environment and Climate\" \/>\n<meta property=\"article:published_time\" content=\"2019-11-08T11:33:43+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-04-04T08:31:08+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/danielle_kristine_image005.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"2048\" \/>\n\t<meta property=\"og:image:height\" content=\"1365\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"mes008\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"mes008\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/2019\\\/11\\\/08\\\/always-be-afraid-of-dna-contamination\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/2019\\\/11\\\/08\\\/always-be-afraid-of-dna-contamination\\\/\"},\"author\":{\"name\":\"mes008\",\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/#\\\/schema\\\/person\\\/8ca571fbe6eeb50de6ef7ee495e02eb7\"},\"headline\":\"Always be afraid of DNA contamination!\",\"datePublished\":\"2019-11-08T11:33:43+00:00\",\"dateModified\":\"2023-04-04T08:31:08+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/2019\\\/11\\\/08\\\/always-be-afraid-of-dna-contamination\\\/\"},\"wordCount\":614,\"image\":{\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/2019\\\/11\\\/08\\\/always-be-afraid-of-dna-contamination\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/wp-content\\\/uploads\\\/sites\\\/492\\\/2019\\\/11\\\/danielle_kristine_image005.jpg\",\"articleSection\":[\"Calypso coring in the Fram Strait (19.3)\",\"Cruise blog\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/2019\\\/11\\\/08\\\/always-be-afraid-of-dna-contamination\\\/\",\"url\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/2019\\\/11\\\/08\\\/always-be-afraid-of-dna-contamination\\\/\",\"name\":\"Always be afraid of DNA contamination! - CAGE, Centre for Arctic Gas Hydrate, Environment and Climate\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/2019\\\/11\\\/08\\\/always-be-afraid-of-dna-contamination\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/2019\\\/11\\\/08\\\/always-be-afraid-of-dna-contamination\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/wp-content\\\/uploads\\\/sites\\\/492\\\/2019\\\/11\\\/danielle_kristine_image005.jpg\",\"datePublished\":\"2019-11-08T11:33:43+00:00\",\"dateModified\":\"2023-04-04T08:31:08+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/#\\\/schema\\\/person\\\/8ca571fbe6eeb50de6ef7ee495e02eb7\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/site.uit.no\\\/cage\\\/2019\\\/11\\\/08\\\/always-be-afraid-of-dna-contamination\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/2019\\\/11\\\/08\\\/always-be-afraid-of-dna-contamination\\\/#primaryimage\",\"url\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/wp-content\\\/uploads\\\/sites\\\/492\\\/2019\\\/11\\\/danielle_kristine_image005.jpg\",\"contentUrl\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/wp-content\\\/uploads\\\/sites\\\/492\\\/2019\\\/11\\\/danielle_kristine_image005.jpg\",\"width\":2048,\"height\":1365,\"caption\":\"DNA sampling requires that scientists follow a strict no-contamination protocol, including the blue paper suits.\"},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/#website\",\"url\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/\",\"name\":\"CAGE, Centre for Arctic Gas Hydrate, Environment and Climate\",\"description\":\"UiT The Arctic University of Norway\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/#\\\/schema\\\/person\\\/8ca571fbe6eeb50de6ef7ee495e02eb7\",\"name\":\"mes008\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/41490ca776111aa67719f2c10bc540d1285a0fb2bd364132fdd4a5603630ab48?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/41490ca776111aa67719f2c10bc540d1285a0fb2bd364132fdd4a5603630ab48?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/41490ca776111aa67719f2c10bc540d1285a0fb2bd364132fdd4a5603630ab48?s=96&d=mm&r=g\",\"caption\":\"mes008\"},\"url\":\"https:\\\/\\\/site.uit.no\\\/cage\\\/author\\\/mariana-esteves\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Always be afraid of DNA contamination! - CAGE, Centre for Arctic Gas Hydrate, Environment and Climate","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/","og_locale":"en_US","og_type":"article","og_title":"Always be afraid of DNA contamination! - CAGE, Centre for Arctic Gas Hydrate, Environment and Climate","og_description":"Text and photos: Danielle Grant and Kristine Steinsland, NORCE Climate Danielle and Kristine here! We &hellip; Read more \u00abAlways be afraid of DNA contamination!\u00bb","og_url":"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/","og_site_name":"CAGE, Centre for Arctic Gas Hydrate, Environment and Climate","article_published_time":"2019-11-08T11:33:43+00:00","article_modified_time":"2023-04-04T08:31:08+00:00","og_image":[{"width":2048,"height":1365,"url":"https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/danielle_kristine_image005.jpg","type":"image\/jpeg"}],"author":"mes008","twitter_card":"summary_large_image","twitter_misc":{"Written by":"mes008","Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/#article","isPartOf":{"@id":"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/"},"author":{"name":"mes008","@id":"https:\/\/site.uit.no\/cage\/#\/schema\/person\/8ca571fbe6eeb50de6ef7ee495e02eb7"},"headline":"Always be afraid of DNA contamination!","datePublished":"2019-11-08T11:33:43+00:00","dateModified":"2023-04-04T08:31:08+00:00","mainEntityOfPage":{"@id":"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/"},"wordCount":614,"image":{"@id":"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/#primaryimage"},"thumbnailUrl":"https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/danielle_kristine_image005.jpg","articleSection":["Calypso coring in the Fram Strait (19.3)","Cruise blog"],"inLanguage":"en-US"},{"@type":"WebPage","@id":"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/","url":"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/","name":"Always be afraid of DNA contamination! - CAGE, Centre for Arctic Gas Hydrate, Environment and Climate","isPartOf":{"@id":"https:\/\/site.uit.no\/cage\/#website"},"primaryImageOfPage":{"@id":"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/#primaryimage"},"image":{"@id":"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/#primaryimage"},"thumbnailUrl":"https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/danielle_kristine_image005.jpg","datePublished":"2019-11-08T11:33:43+00:00","dateModified":"2023-04-04T08:31:08+00:00","author":{"@id":"https:\/\/site.uit.no\/cage\/#\/schema\/person\/8ca571fbe6eeb50de6ef7ee495e02eb7"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/site.uit.no\/cage\/2019\/11\/08\/always-be-afraid-of-dna-contamination\/#primaryimage","url":"https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/danielle_kristine_image005.jpg","contentUrl":"https:\/\/site.uit.no\/cage\/wp-content\/uploads\/sites\/492\/2019\/11\/danielle_kristine_image005.jpg","width":2048,"height":1365,"caption":"DNA sampling requires that scientists follow a strict no-contamination protocol, including the blue paper suits."},{"@type":"WebSite","@id":"https:\/\/site.uit.no\/cage\/#website","url":"https:\/\/site.uit.no\/cage\/","name":"CAGE, Centre for Arctic Gas Hydrate, Environment and Climate","description":"UiT The Arctic University of Norway","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/site.uit.no\/cage\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/site.uit.no\/cage\/#\/schema\/person\/8ca571fbe6eeb50de6ef7ee495e02eb7","name":"mes008","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/41490ca776111aa67719f2c10bc540d1285a0fb2bd364132fdd4a5603630ab48?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/41490ca776111aa67719f2c10bc540d1285a0fb2bd364132fdd4a5603630ab48?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/41490ca776111aa67719f2c10bc540d1285a0fb2bd364132fdd4a5603630ab48?s=96&d=mm&r=g","caption":"mes008"},"url":"https:\/\/site.uit.no\/cage\/author\/mariana-esteves\/"}]}},"amp_enabled":true,"_links":{"self":[{"href":"https:\/\/site.uit.no\/cage\/wp-json\/wp\/v2\/posts\/3962","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/site.uit.no\/cage\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/site.uit.no\/cage\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/site.uit.no\/cage\/wp-json\/wp\/v2\/users\/123546"}],"replies":[{"embeddable":true,"href":"https:\/\/site.uit.no\/cage\/wp-json\/wp\/v2\/comments?post=3962"}],"version-history":[{"count":1,"href":"https:\/\/site.uit.no\/cage\/wp-json\/wp\/v2\/posts\/3962\/revisions"}],"predecessor-version":[{"id":3966,"href":"https:\/\/site.uit.no\/cage\/wp-json\/wp\/v2\/posts\/3962\/revisions\/3966"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/site.uit.no\/cage\/wp-json\/wp\/v2\/media\/3963"}],"wp:attachment":[{"href":"https:\/\/site.uit.no\/cage\/wp-json\/wp\/v2\/media?parent=3962"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/site.uit.no\/cage\/wp-json\/wp\/v2\/categories?post=3962"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/site.uit.no\/cage\/wp-json\/wp\/v2\/tags?post=3962"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}