The class I pancreatic elastase from Atlantic salmon is\u00a0considered to be a cold-adapted enzyme in view of the cold habitat, the\u00a0reduced thermostability of the enzyme, and the fact that it is faster than\u00a0its mesophilic porcine counterpart at room temperature. However, no\u00a0experimental characterization of its catalytic properties at lower\u00a0temperatures has actually been reported. Here we use extensive computer\u00a0simulations of its catalytic reaction, at diff erent temperatures and with\u00a0diff erent peptide substrates, to compare its characteristics with those of\u00a0porcine pancreatic elastase, with which it shares 67% sequence identity.\u00a0We fi nd that both enzymes have a preference for smaller aliphatic\u00a0residues at the P1 position, while the reaction rate with phenylalanine at\u00a0P1 is predicted to be substantially lower. With the former class of\u00a0substrates, the calculated reaction rates for salmon enzyme are\u00a0consistently higher than those of the porcine ortholog at all temperatures examined, and the diff erence is most pronounced\u00a0at the lowest temperature. As observed for other cold-adapted enzymes, this is caused by redistribution of the activation free\u00a0energy in terms of enthalpy and entropy and can be linked to diff erences in the mobility of surface-exposed loops in the two\u00a0enzymes. Such mobility changes are found to be refl ected by characteristic sequence conservation patterns in psychrophilic and\u00a0mesophilic species. Hence, calculations of mutations in a single surface loop show that the temperature dependence of the\u00a0catalytic reaction is altered in a predictable way.<\/p>\n
<\/p>\n
Read the paper<\/a><\/p>\n","protected":false},"excerpt":{"rendered":" Catalytic Adaptation of Psychrophilic Elastase The class I pancreatic elastase from Atlantic salmon is\u00a0considered to be a cold-adapted enzyme in view of the cold habitat, the\u00a0reduced thermostability of the enzyme, and the fact that it is faster than\u00a0its mesophilic porcine … Continued<\/a><\/p>\n","protected":false},"author":862,"featured_media":266,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[8,9,7],"class_list":["post-265","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications","tag-cold-adaptation","tag-elastase","tag-publication"],"_links":{"self":[{"href":"https:\/\/site.uit.no\/cube\/wp-json\/wp\/v2\/posts\/265","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/site.uit.no\/cube\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/site.uit.no\/cube\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/site.uit.no\/cube\/wp-json\/wp\/v2\/users\/862"}],"replies":[{"embeddable":true,"href":"https:\/\/site.uit.no\/cube\/wp-json\/wp\/v2\/comments?post=265"}],"version-history":[{"count":1,"href":"https:\/\/site.uit.no\/cube\/wp-json\/wp\/v2\/posts\/265\/revisions"}],"predecessor-version":[{"id":267,"href":"https:\/\/site.uit.no\/cube\/wp-json\/wp\/v2\/posts\/265\/revisions\/267"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/site.uit.no\/cube\/wp-json\/wp\/v2\/media\/266"}],"wp:attachment":[{"href":"https:\/\/site.uit.no\/cube\/wp-json\/wp\/v2\/media?parent=265"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/site.uit.no\/cube\/wp-json\/wp\/v2\/categories?post=265"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/site.uit.no\/cube\/wp-json\/wp\/v2\/tags?post=265"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}