Technical development:
- A dedicated semi-automated experimental setup has been developed for NanoPath project.
- New design, optimization and loss measurement for BIOTEK photonic chips have been completed.
- Custom-made LabView interface has been developed for illumination and collection of the fluorescent signal for chip-based TIRF, IFON and SMLM.
- Fluorescence labelling has been optimized for Tokuyasu sections.
Samples investigated under BioTek 2021 project: the table below illustrates diverse tissue samples imaged on-chip. We have performed multicolor fluorescence imaging on all samples.
| Preservation method | Species | Sample type | Source | On-chip imaging method | |||
| TIRF | MUSICAL | SMLM | CLEM | ||||
| Tokuyasu | Human | Placenta | UNN / UiT | x | x | ||
| Mouse | Kidney | UiT | x | x | |||
| Pig | Heart | UiT | x | ||||
| Zebra fish | Eye | UZH | x | x | x | ||
| FFPE | Human | Placenta | Karolinska Institute | x | |||
| Human | CRC | Radium Hospital | x | ||||
| Human | Lung | Radium Hospital | x | ||||
| Human | Prostate | Radium Hospital | x | ||||