{"id":111,"date":"2021-06-02T16:21:27","date_gmt":"2021-06-02T14:21:27","guid":{"rendered":"https:\/\/site.uit.no\/nanopath\/?page_id=111"},"modified":"2023-02-20T15:18:29","modified_gmt":"2023-02-20T14:18:29","slug":"111-2","status":"publish","type":"page","link":"https:\/\/site.uit.no\/nanopath\/111-2\/","title":{"rendered":"News"},"content":{"rendered":"<h2><strong>Technical development:<\/strong><\/h2>\n<ol>\n<li>A dedicated semi-automated experimental setup has been developed for\u00a0NanoPath\u00a0project.<\/li>\n<li>New design, optimization and loss measurement for BIOTEK photonic chips have been completed.<\/li>\n<li>Custom-made LabView interface\u00a0has been developed\u00a0for illumination and collection of the fluorescent signal\u00a0for chip-based TIRF, IFON and SMLM.<\/li>\n<li>Fluorescence labelling has been optimized for\u00a0Tokuyasu\u00a0sections.<\/li>\n<\/ol>\n<p>Samples investigated\u00a0under\u00a0BioTek\u00a02021 project: the table below illustrates diverse tissue\u00a0samples imaged\u00a0on-chip. We have performed multicolor fluorescence imaging on all samples.<\/p>\n<table style=\"font-weight: 400\">\n<tbody>\n<tr>\n<td colspan=\"1\" rowspan=\"2\"><b>Preservation\u00a0method<\/b><\/td>\n<td colspan=\"1\" rowspan=\"2\"><b>Species<\/b><\/td>\n<td colspan=\"1\" rowspan=\"2\"><b>Sample type<\/b><\/td>\n<td colspan=\"1\" rowspan=\"2\"><b>Source<\/b><\/td>\n<td style=\"text-align: left\" colspan=\"4\"><b>\u00a0 \u00a0On-chip\u00a0imaging\u00a0method<\/b><\/td>\n<\/tr>\n<tr>\n<td><b>TIRF<\/b><\/td>\n<td><b>MUSICAL<\/b><\/td>\n<td><b>SMLM<\/b><\/td>\n<td><b>CLEM<\/b><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"4\"><span style=\"color: #0000ff\">Tokuyasu<\/span><\/td>\n<td><span style=\"color: #0000ff\">Human<\/span><\/td>\n<td><span style=\"color: #0000ff\">Placenta<\/span><\/td>\n<td><span style=\"color: #0000ff\">UNN \/ UiT<\/span><\/td>\n<td><span style=\"color: #0000ff\">\u00a0 x<\/span><\/td>\n<td><span style=\"color: #0000ff\">\u00a0 \u00a0 \u00a0x<\/span><\/td>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #0000ff\">Mouse<\/span><\/td>\n<td><span style=\"color: #0000ff\">Kidney<\/span><\/td>\n<td><span style=\"color: #0000ff\">UiT<\/span><\/td>\n<td><span style=\"color: #0000ff\">\u00a0 x<\/span><\/td>\n<td><\/td>\n<td><span style=\"color: #0000ff\">\u00a0 \u00a0 x<\/span><\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #0000ff\">Pig<\/span><\/td>\n<td><span style=\"color: #0000ff\">Heart<\/span><\/td>\n<td><span style=\"color: #0000ff\">UiT<\/span><\/td>\n<td><span style=\"color: #0000ff\">\u00a0 x<\/span><\/td>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #0000ff\">Zebra\u00a0fish<\/span><\/td>\n<td><span style=\"color: #0000ff\">Eye<\/span><\/td>\n<td><span style=\"color: #0000ff\">UZH<\/span><\/td>\n<td><span style=\"color: #0000ff\">\u00a0 x<\/span><\/td>\n<td><span style=\"color: #0000ff\">\u00a0 \u00a0 \u00a0x<\/span><\/td>\n<td><\/td>\n<td><span style=\"color: #0000ff\">\u00a0 \u00a0 x<\/span><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"4\"><span style=\"color: #800000\">FFPE<\/span><\/td>\n<td><span style=\"color: #800000\">Human<\/span><\/td>\n<td><span style=\"color: #800000\">Placenta<\/span><\/td>\n<td><span style=\"color: #800000\">Karolinska\u00a0Institute<\/span><\/td>\n<td><span style=\"color: #800000\">\u00a0 x<\/span><\/td>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #800000\">Human<\/span><\/td>\n<td><span style=\"color: #800000\">CRC<\/span><\/td>\n<td><span style=\"color: #800000\">Radium Hospital<\/span><\/td>\n<td><span style=\"color: #800000\">\u00a0 x<\/span><\/td>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #800000\">Human<\/span><\/td>\n<td><span style=\"color: #800000\">Lung<\/span><\/td>\n<td><span style=\"color: #800000\">Radium Hospital<\/span><\/td>\n<td><span style=\"color: #800000\">\u00a0 x<\/span><\/td>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #800000\">Human<\/span><\/td>\n<td><span style=\"color: #800000\">Prostate<\/span><\/td>\n<td><span style=\"color: #800000\">Radium Hospital<\/span><\/td>\n<td><span style=\"color: #800000\">\u00a0 x<\/span><\/td>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Technical development: A dedicated semi-automated experimental setup has been developed for\u00a0NanoPath\u00a0project. New design, optimization and loss measurement for BIOTEK photonic chips have been completed. Custom-made LabView interface\u00a0has been developed\u00a0for illumination and collection of the fluorescent signal\u00a0for chip-based TIRF, IFON and &hellip; <a href=\"https:\/\/site.uit.no\/nanopath\/111-2\/\">Continued<\/a><\/p>\n","protected":false},"author":123470,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-111","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/site.uit.no\/nanopath\/wp-json\/wp\/v2\/pages\/111","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/site.uit.no\/nanopath\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/site.uit.no\/nanopath\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/site.uit.no\/nanopath\/wp-json\/wp\/v2\/users\/123470"}],"replies":[{"embeddable":true,"href":"https:\/\/site.uit.no\/nanopath\/wp-json\/wp\/v2\/comments?post=111"}],"version-history":[{"count":14,"href":"https:\/\/site.uit.no\/nanopath\/wp-json\/wp\/v2\/pages\/111\/revisions"}],"predecessor-version":[{"id":185,"href":"https:\/\/site.uit.no\/nanopath\/wp-json\/wp\/v2\/pages\/111\/revisions\/185"}],"wp:attachment":[{"href":"https:\/\/site.uit.no\/nanopath\/wp-json\/wp\/v2\/media?parent=111"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}